What is Amniotic Fluid?
The amniotic fluid is found in the amniotic sac, it surrounds the developing human embryo. It was previously considered to be just fetal urine.  Then, for more than 50 years, human amniotic fluid cells were used just as a tool for the prenatal diagnosis of fetal genetic aberrations. However, nowadays, people are starting to realize the true potential of amniotic fluid which was previously untapped.  Amniotic fluid possesses immunomodulatory properties. Furthermore, it does not have the ethical and legal limitations of embryonic stem cells. The use of amniotic fluid in regenerative medicine is rather recent.  Amniotic fluid comprises of water, electrolytes, lipids, proteins, various growth factors, cytokines, hormones and pluripotent cells that can be used in regenerative medicine for purposes like tissue regeneration, transplantation and therapy. This may offer a long-term solution to the problems of organ shortage in transplantation, as these pluripotent cells are capable of differentiating into different lines of cells, e.g. adipocytes, osteocytes and fibroblasts.
What is PRP?
The human blood contains different component cells (e.g. white blood cells, red blood cells), cell fragments (e.g. platelets), liquid (i.e. plasma) and various growth factors (e.g. VEGF, IGF, PDGF, TGF-β, et cetera) and cytokines. When the blood is spun in a centrifuge in a specific method, it removes the red blood cells (RBCs) and concentrates the platelets in the plasma.  This plasma has an abundance of platelets, and is rightly named, platelet-rich plasma. PRP has 1,000,000 platelets per micro liter of blood in contrast to normal range between 150,000 and 350,000 per micro liter of blood. Platelet-rich plasma, once formed, is stored in an anticoagulant.
Using Amniotic Fluid with PRP
According to a study conducted by Kermanshah University of Medical Sciences on rabbits, in which one group was injected with just platelet-rich plasma while the other was injected with both platelet-rich plasma and amniotic fluid, overall findings demonstrated that human amniotic fluid in combination with PRP had greater influence on enhancing bone formation in rabbit’s dental socket preservation. The significant difference that was found between PRP alone versus combination of amniotic fluid and platelet-rich plasma might be due to the conjunction of FGF and presented HA in amniotic fluid. As reported by Lisignoli et al., human mesenchymal stem cells differentiation in HA scaffold is significantly enhanced by introducing b-FGF.
The beneficial outcomes of applying PRP, including reduction of bleeding and rapid healing, holds great promise for dental clinic procedures. According to experimental and clinical studies, it is widely accepted that stem cells-loaded PRP gel can be directly used to restore various bone and cartilage defect in the restoration of large alveolar defect. 
Moreover, it is widely accepted that there is a positive, synergistic effect of PRP on the regenerative effect of amniotic fluid.
This treatment is an excellent add-on to any clinic as people have started realizing its true potential. It is becoming an increasingly popular treatment to improve the texture, tone and firmness of the skin. PRP also helps to reduce fine lines and wrinkles.
- It can be used to treat the delicate areas above and below the eyes which are usually difficult to treat with treatments such as Botox and dermal fillers.
- Other than aesthetic, platelet-rich plasma is used by rheumatologists in the treatment of various age-related and sports-related injuries.
- This treatment is cost effective as it incurs very little outlays, making it a very lucrative procedure.
- Platelet-rich plasma is an autologous product, thus, patients who are opposed to using artificial products will be happier to try this treatment as it is completely natural and safe.
- Patients see long-lasting results.
- Very little down time for patients
- Application of platelet-rich plasma also enhances soft tissue healing and speeds up epithelialization. The soft tissue-healing enhancement is recorded in palatal grafts, gingival flaps, and cosmetic dentistry soft tissue augmentations.
Amniotic Fluid Training
Using amniotic fluid for regenerative medicine is completely ethical as it does not cause harm to either the baby or the mother
- The collection of amniotic fluid in full-term cesarean deliveries adds on average just 90 seconds to the operation.
- The amniotic stem cells are non-teratogenic.
- Due to the pluripotent properties of cells in amniotic fluid and their ability to engraft in injured organs and modulate immune and repair responses of host tissues, suggest that transplantation of such cells may be useful for the treatment of various degenerative and inflammatory diseases.
- Amniotic fluid treatment is ideal for use in orthopedics and treatment of cardiovascular disease 
- High levels of cytokines can be exploited for controlling immune responses in arthritis. Amniotic fluid (AF) also contains various kinds of growth factors and can be an excellent source of initiating and supporting wound healing. If given during a surgery, amniotic fluid can support early healing of patient.
- Use of amniotic fluid during an organ transplantation can be beneficial, just as it would also be for hair regeneration and other aesthetic purposes.
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 Loukogeorgakis, SP, Coppi De, P. Stem cells from amniotic fluid – Potential for regenerative medicine. Best Pract Res Clin Obstet Gynaecol 2015; 44: 1– 13.
 Loukogeorgakis, SP, Maghsoudlou, P,Coppi, P. Recent developments in therapies with stem cells from amniotic fluid and placenta. Fetal Maternal Med Rev2013; 24: 148– 168.
 Cananzi, M, Atala, A, De Coppi, P. Stem cells derived from amniotic fluid: new potentials in regenerative medicine. Reprod Biomed Online 2009 18(Suppl 1): 17–27.
 Barrientos S., Stojadinovic O., Golinko M.S., Brem H., Tomic-Canic M.. Growth factors and cytokines in wound healing. Wound Repair Regen. 2008;16:585–601.
 Hsu W.K, Mishra A., Rodeo S.R., Fu F., Terry M.A., Randelli P.. et al. Platelet-rich plasma in orthopaedic applications: evidence-based recommendations for treatment. J.o Am. Acad. Orthop. Surg. 2013;21:739–748.
 Lisignoli G, Fini M, Giavaresi G, Nicoli AN, Toneguzzi S, Acchini A. Osteogenesis of large segmental radius defects enhanced by basic fibroblast growth factor activated bone marrow stromal cells grown on non-woven hyaluronic acid-based polymer scaffold. Biomaterials 2002;23:1043-51.
 Haleem, A. M., Singergy, A. A. E., et al. The Clinical Use of Human Culture–Expanded Autologous Bone Marrow Mesenchymal Stem Cells Transplanted on Platelet-Rich Fibrin Glue in the Treatment of Articular Cartilage Defects: A Pilot Study and Preliminary Results. Sage Journals 2010; 1(4): 253-261.
 Bollini, S, Cheung, KK, Riegler, J, Dong, X, Smart, N, Ghionzoli, M, et al. Amniotic fluid stem cells are cardioprotective following acute myocardial infarction. Stem Cells Dev 2011 Nov; 20(11): 1985–94.